Teste Repositóri
2014-01-01
Resultados de pesquisa
Foram encontrados 24 registos.
As bactérias são procariotas que pertencem ao domínio Bacteria e apresentam três formas básicas: esférica, cilíndrica e helicoidal. As células podem associar-se, formando arranjos característicos de alguns géneros bacterianos. A parede celular, além de conferir forma às bactérias, é responsável pela diferenciação entre bactérias Gram-positivo e Gram-negativo. Além disso, é um alvo preferencial para a atuação de alguns antibióticos.
As bactérias patogénicas para o Homem obtêm a sua energia pelo catabolismo de diferentes tipos de nutrientes. Esta energia é utilizada, por exemplo, para a mobilidade bacteriana e para a síntese de macromoléculas. O catabolismo dos açúcares (pentoses e hexoses) ocorre essencialmente através das vias de Embden-Meyerof-Parnas, das pentoses-fosfato e de Entner-Doudoroff. O NADH e o NADPH produzidos nestes proce...
Os micoplasmas, organismos que integram a classe Mollicutis, constituem um vasto grupo de procariotas que se diferenciam das outras bactérias pelo seu pequeno tamanho celular, pela falta de parede celular e pelas dimensões reduzidas do seu genoma. Mycoplasma e Ureoplasma são os géneros com maior importância médica, com algumas das suas espécies envolvidas em pneumonias, infeções urinárias e artrites no Homem. A natural associação dos micoplasmas com os seus hospedeiros específicos e o estado do sistema imunológico dos mesmos constituem fatores importantes na disseminação e estabelecimento das micoplasmoses, tornando difícil a sua prevenção e controlo. Na identificação dos micoplasmas em cultura ou em amostras clínicas podem ser utlizados testes serológicos (imunofluorescência, fixação do complemento, imunoenzimáticos e moleculares
Poster presented at the 7th iMed.ULisboa Postgraduate Students Meeting. Lisbon, 15 July 2015
Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004.Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic, and the household of a nanny. Adenovirus infection was confirmed in 48 children by PCR and virus isolation. Most(96%) isolates were classified as being of subspecies B1. Phylogenetic analysis of fiber and hexon gene sequences revealed that most infants were infected with AdV serotype 3 (AdV3) strains. Infants attending one nursery harbored a new recombinant strain containing an AdV serotype 7 hexon and serotype 3 fiber (AdV7/3). Both the AdV3 and the AdV7/3 strains caused fatal infections. Two different serotype 3 strains ...
HIV-2 infection was diagnosed in two patients 15 and 24 years of age and, thereafter, in their mothers. Epidemiological data suggested that vertical transmission was the most probable mode of infection in both patients (Mota-Miranda A, et al.: AIDS 2001;
In the present study, we sought to define the importance of serum IgA (sIgA)-mediated immunity in HIV-2 infection. Serum samples from a total of 29 HIV-2-infected patients from Guinea-Bissau (n = 20) and Portugal (n = 9) were studied. Samples from seronegative individuals were used as controls. Antibody reactivity to native and recombinant envelope glycoproteins as well as peptides representing various regions of the envelope glycoproteins was investigated. Furthermore, the capacity of purified IgA to neutralize the HIV-2(SBL6669) strain was tested. All serum samples showed IgA reactivity against whole HIV-2 antigen. Twenty-eight out of 29 IgA samples (96%) reacted with native HIV-2 gp125, 26/29 (90%) with recombinant gp105, and 29/29 (100%) with recombinant gp36. When using peptides, the most prominent IgA reactivity was seen against ...
The viral load assays AMPLICOR HIV-1 Monitor Test 1.5, Nuclisens HIV-1 QT, and Quantiplex HIV RNA 3.0 (bDNA) were evaluated for their abilities to quantify human immunodeficiency virus type 1 (HIV-1) RNA in 64 plasma samples from 21 children infected in P
The study of true seronegative HIV-1 infections may have important implications for the diagnosis and prevention of HIV-1 infection. The case of an AIDS patient with persistently negative HIV serology is described. Genetic and phylogenetic analysis indica
