Teste Repositóri
2014-01-01
Resultados de pesquisa
Foram encontrados 10 registos.
Using a green methodology, 17 different poly(2-oxazolines) were synthesized starting from four different oxazoline monomers. The polymerization reactions were conducted in
supercritical carbon dioxide under a cationic ring-opening polymerization (CROP)
mechanism using boron trifluoride diethyl etherate as the catalyst. The obtained living
polymers were then end-capped with different types of amines, in order to confer them
antimicrobial activity. For comparison, four polyoxazolines were end-capped with water, and by their hydrolysis the linear poly(ethyleneimine) (LPEI) was also produced.
After functionalization the obtained polymers were isolated, purified and characterized by standard techniques (FT-IR, NMR, MALDI-TOF and GPC).
The synthesized poly(2-oxazolines) revealed an unusual intrinsic blue photoluminescence.
High concen...
A vergonha tem despertado um crescente interesse na comunidade científica devido à sua forte associação com diferentes indicadores de psicopatologia e de qualidade de vida. Uma das características percepcionada como alvo de avaliação negativa dos outros é a aparência física. De facto, a literatura tem documentado a associação entre insatisfação corporal e as experiências de vergonha, associação esta que se assume ainda mais proeminentes em mulheres com excesso de peso. No entanto, é possível que processos de regulação emocional possam mediar o impacto de uma auto-avaliação negativa associada à imagem corporal e o grau de vergonha externa. O presente estudo explora o papel mediador da fusão cognitiva com a imagem corporal na relação entre variáveis de insatisfação corporal e de comparações desfavoráveis baseadas na imagem corporal e a v...
Tese de doutoramento, Ciências e Tecnologias da Saúde (Microbiologia), Universidade de Lisboa, Faculdade de Medicina, 2018
A research project on antiviral drug resistance of influenza viruses circulating in Portugal has been carried out since 2007. Here, the first results obtained regarding the evaluation of susceptibility to amantadine and oseltamivir are presented. Information about antiviral prescription and exposure was available through the National Influenza Surveillance Programme. Amantadine susceptibility was evaluated by
pyrosequencing for known resistance markers on 178 influenza A strains from 2004/2005 to 2006/2007. Susceptibility to oseltamivir was evaluated by 50% inhibitory concentration determination on 340 virus strains from 2004/2005 to 2008/2009, 134 of which were further analyzed by sequencing of the neuraminidase gene. This study revealed that influenza antiviral drugs were rarely prescribed at national level. Resistance to amantadine...
A research project on antiviral drug resistance of influenza viruses circulating in Portugal has been carried out since 2007. Here, the first results obtained regarding the evaluation of susceptibility to amantadine and oseltamivir are presented. Information about antiviral prescription and exposure was available through the National Influenza Surveillance Programme. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 178 influenza A strains from 2004/2005 to 2006/2007. Susceptibility to oseltamivir was evaluated by 50% inhibitory concentration determination on 340 virus strains from 2004/2005 to 2008/2009, 134 of which were further analyzed by sequencing of the neuraminidase gene. This study revealed that influenza antiviral drugs were rarely prescribed at national level. Resistance to amantadine ...
BACKGROUND: Genetic changes in influenza surface and internal genes can alter viral fitness and virulence. Mutation trend analysis and antiviral drug susceptibility profiling of A(H1N1)pdm09 viruses is essential for risk assessment of emergent strains and disease management.
OBJECTIVE: To profile genomic signatures and antiviral drug resistance of A(H1N1)pdm09 viruses and to discuss the potential role of mutated residues in human host adaptation and virulence.
STUDY DESIGN: A(H1N1)pdm09 viruses circulating in Portugal during pandemic and post-pandemic periods and 2009/2010 season. Viruses were isolated in MDCK-SIAT1 cell culture and subjected to mutation analysis of surface and internal proteins, and to antiviral drug susceptibility profiling.
RESULTS: The A(H1N1)pdm09 strains circulating during the epidemic period in Portugal...
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Background: The search for new antimicrobial compounds able to overcome the global issue of microbial resistance
to antibiotics is a priority worldwide. Moreover, several commensal microorganisms have been increasingly associated
to opportunistic microbial infections. Having previously disclosed the green synthesis and preliminary characterization
of the oligomers [linear oligo(ethylenimine) hydrochloride and oligo(2-methyl-2-oxazoline) quaternized with N,Ndimethyldodecylamine]
we herein report on the screening of these oligomers against a battery of 69 clinical isolates
of Aerococcus spp., Candida spp., Staphylococcus spp. and Streptococcus spp.
Findings: The isolates’ susceptibility to both oligomers was evaluated by determining their minimal inhibitory concentration
(MIC) and the biocidal effectiveness of each compound was further c...
